cAMP-bound SpSLC9C1 in lipid nanodiscs, dimer
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8pd8
Q-score: 0.378
Kalienkova V, Peter MF, Rheinberger J, Paulino C
Nature (2023) 623 pp. 202-209 [ DOI: doi:10.1038/s41586-023-06629-w Pubmed: 37880361 ]
- Spslc9c1 (300 kDa, Complex from Strongylocentrotus purpuratus)
- Adenosine-3',5'-cyclic-monophosphate (329 Da, Ligand)
- Sperm-specific sodium proton exchanger (147 kDa, Protein from Strongylocentrotus purpuratus)
cAMP-bound SpSLC9C1 in lipid nanodiscs, protomer state 1
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8pd9
Q-score: 0.393
Kalienkova V, Peter MF, Rheinberger J, Paulino C
Nature (2023) 623 pp. 202-209 [ DOI: doi:10.1038/s41586-023-06629-w Pubmed: 37880361 ]
- Spslc9c1 (300 kDa, Complex from Strongylocentrotus purpuratus)
- Adenosine-3',5'-cyclic-monophosphate (329 Da, Ligand)
- Sperm-specific sodium proton exchanger (147 kDa, Protein from Strongylocentrotus purpuratus)
cAMP-bound SpSLC9C1 in lipid nanodiscs, protomer state 2
Kalienkova V, Peter MF, Rheinberger J, Paulino C
Nature (2023) 623 pp. 202-209 [ DOI: doi:10.1038/s41586-023-06629-w Pubmed: 37880361 ]
- Spslc9c1 (300 kDa, Complex from Strongylocentrotus purpuratus)
- Spslc9c1 (Protein from Strongylocentrotus purpuratus)
Cryo-EM structure of the human Sec61 complex in a partially-open apo state (Class 1)
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8dnv
Q-score: 0.519
Itskanov S, Wang L, Junne T, Sherriff R, Xiao L, Blanchard N, Shi WQ, Forsyth C, Hoepfner D, Spiess M, Park E
Nat Chem Biol (2023) 19 pp. 1063-1071 [ Pubmed: 37169959 DOI: doi:10.1038/s41589-023-01337-y ]
- A human-yeast chimeric sec complex (Complex from Homo sapiens)
- Protein transport protein sec61 subunit beta (9 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit alpha isoform 1 (52 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Homo sapiens)
Cryo-EM structure of the human Sec61 complex in a partially-open apo state (Class 2)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8dnw
Q-score: 0.467
Itskanov S, Wang L, Junne T, Sherriff R, Xiao L, Blanchard N, Shi WQ, Forsyth C, Hoepfner D, Spiess M, Park E
Nat Chem Biol (2023) 19 pp. 1063-1071 [ Pubmed: 37169959 DOI: doi:10.1038/s41589-023-01337-y ]
- A human-yeast chimeric sec complex (Complex from Homo sapiens)
- Protein transport protein sec61 subunit beta (9 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit alpha isoform 1 (52 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Homo sapiens)
Cryo-EM structure of the human Sec61 complex inhibited by cotransin
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8dnx
Q-score: 0.465
Itskanov S, Wang L, Junne T, Sherriff R, Xiao L, Blanchard N, Shi WQ, Forsyth C, Hoepfner D, Spiess M, Park E
Nat Chem Biol (2023) 19 pp. 1063-1071 [ Pubmed: 37169959 DOI: doi:10.1038/s41589-023-01337-y ]
- Cotransin analogue peptide inhibitor (953 Da, Protein from synthetic construct)
- A human-yeast chimeric sec complex treated with cotransin (Complex from Homo sapiens)
- Protein transport protein sec61 subunit beta (9 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit alpha isoform 1 (52 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Homo sapiens)
Cryo-EM structure of the human Sec61 complex inhibited by decatransin
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 8dny
Q-score: 0.546
Itskanov S, Wang L, Junne T, Sherriff R, Xiao L, Blanchard N, Shi WQ, Forsyth C, Hoepfner D, Spiess M, Park E
Nat Chem Biol (2023) 19 pp. 1063-1071 [ Pubmed: 37169959 DOI: doi:10.1038/s41589-023-01337-y ]
- Protein transport protein sec61 subunit beta (9 kDa, Protein from Homo sapiens)
- A human-yeast chimeric sec complex treated with decatransin (Complex from Homo sapiens)
- Decatransin peptide inhibitor (1 kDa, Protein from synthetic construct)
- Protein transport protein sec61 subunit alpha isoform 1 (52 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Homo sapiens)
Cryo-EM structure of the human Sec61 complex inhibited by apratoxin F
Resolution: 2.57 Å
EM Method: Single-particle
Fitted PDBs: 8dnz
Q-score: 0.602
Itskanov S, Wang L, Junne T, Sherriff R, Xiao L, Blanchard N, Shi WQ, Forsyth C, Hoepfner D, Spiess M, Park E
Nat Chem Biol (2023) 19 pp. 1063-1071 [ Pubmed: 37169959 DOI: doi:10.1038/s41589-023-01337-y ]
- Protein transport protein sec61 subunit beta (9 kDa, Protein from Homo sapiens)
- A human-yeast chimeric sec complex treated with apratoxin f (Complex from Homo sapiens)
- Protein transport protein sec61 subunit alpha isoform 1 (52 kDa, Protein from Homo sapiens)
- Apratoxin f peptide inhibitor (848 Da, Protein from Lyngbya bouillonii)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Homo sapiens)
Cryo-EM structure of the human Sec61 complex inhibited by mycolactone
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 8do0
Q-score: 0.544
Itskanov S, Wang L, Junne T, Sherriff R, Xiao L, Blanchard N, Shi WQ, Forsyth C, Hoepfner D, Spiess M, Park E
Nat Chem Biol (2023) 19 pp. 1063-1071 [ Pubmed: 37169959 DOI: doi:10.1038/s41589-023-01337-y ]
- A human-yeast chimeric sec complex treated with mycolactone (Complex from Homo sapiens)
- Protein transport protein sec61 subunit beta (9 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Protein transport protein sec61 subunit alpha isoform 1 (52 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Homo sapiens)
- [(6~{s},7~{s},9~{z},12~{r})-12-[(~{z},2~{s},6~{r},7~{r},9~{r})-4,6-dimethyl-7,9-bis(oxidanyl)dec-4-en-2-yl]-7,9-dimethyl-2-oxidanylidene-1-oxacyclododec-9-en-6-yl] (2~{e},4~{e},6~{e},8~{e},10~{e},12~{s},13~{s},15~{s})-4,6,10-trimethyl-12,13,15-tris(oxidanyl)hexadeca-2,4,6,8,10-pentaenoate (743 Da, Ligand)
Cryo-EM structure of the human Sec61 complex inhibited by ipomoeassin F
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 8do1
Q-score: 0.511
Itskanov S, Wang L, Junne T, Sherriff R, Xiao L, Blanchard N, Shi WQ, Forsyth C, Hoepfner D, Spiess M, Park E
Nat Chem Biol (2023) 19 pp. 1063-1071 [ Pubmed: 37169959 DOI: doi:10.1038/s41589-023-01337-y ]
- Protein transport protein sec61 subunit beta (9 kDa, Protein from Homo sapiens)
- [(1~{s},3~{r},4~{s},5~{r},6~{r},8~{r},10~{s},23~{r},24~{r},25~{r},26~{r})-5-acetyloxy-6-methyl-4,26-bis(oxidanyl)-17,20-bis(oxidanylidene)-10-pentyl-24-[(~{e})-3-phenylprop-2-enoyl]oxy-2,7,9,21,27-pentaoxatricyclo[21.3.1.0^{3,8}]heptacosan-25-yl] (~{e})-2-methylbut-2-enoate (830 Da, Ligand)
- A human-yeast chimeric sec complex treated with ipomoeassin f (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Protein transport protein sec61 subunit alpha isoform 1 (52 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Homo sapiens)